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Figure 2 | Molecular Cancer

Figure 2

From: The p53-targeting human phosphatase hCdc14A interacts with the Cdk1/cyclin B complex and is differentially expressed in human cancers

Figure 2

Up-regulation of hCdc14A protein expression following treatment with 5aza-dC and/or TSA or MG132. A) Cells were treated with 5aza-dC and TSA alone or in combination and the levels of hCdc14A and MLH1 were analyzed using Western blot and anti-hCdc14A (Ab-2) or anti-MLH1 antibodies. hCdc14A was up-regulated by both 5aza-dC and TSA in the two neuroblastoma cell lines IMR32 and SK-N-SH while in normal fibroblasts and RKO cells hCdc14A levels only responded to TSA. B) Normal fibroblasts (top) or SK-N-SH neuroblastoma cells (bottom) were either UV-irradiated (20 J/m2) or treated for 16 hours with the transcription inhibitor DRB (100 μM), the Cdk and transcription inhibitor roscovitine (50 μM) or the proteasome inhibitor MG132 (10 μM) before the cells were harvested and hCdc14A protein levels analyzed on Western blot using anti-hCdc14A (Ab-1) or anti-actin antibodies (loading control).

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Molecular Cancer

ISSN: 1476-4598