Figure 3
From: Methylation mediated silencing of TMS1/ASC gene in prostate cancer
Expression and methylation pattern of TMS1/ASC gene in drug treated LNCaP cells and in Tumor tissues. Panel A shows restoration of TMS1/ASC gene expression following treatment with 5-Aza but not with TSA alone. Panel B – MS-PCR analysis on the drug treated LNCaP cells confirm expression of TMS1/ASC is associated with partial demethylation of the gene seen as a band on the UM lane in 5-Aza treated cells. Presence of a band in lanes marked as UM indicates presence of unmethylated allele and a band in the lanes marked M denotes a methylated allele. NC-negative control; PC-positive control; UM-unmethylated allele; M-methylated allele; LN-C-untreated LNCaP cells; LN-AZA -5-AZA-2'-deoxycytidine treated cells; LN-AT – 5-AZA-2'-deoxycytidine followed by TSA; LN-TSA – TSA treated cells.