Table 1 Table showing details of primers used and cycling conditions for MS-PCR, RT-PCR and PCR amplification of the CHIP DNA.
From: Methylation mediated silencing of TMS1/ASC gene in prostate cancer
Gene | Primer sequence (5'-3') | Anneal. Temp. (°C) | No. of Cycles |
|---|---|---|---|
TMS1/ASC methylated | F- CGA TTT TGG CGT TTT TCG ACG GTT | 65 | 35 |
| Â | R- CCG CTC ACC CCG CTA CAA CCG C | Â | Â |
TMS1/ASC Unmethylated | F- TTG TTG GAG GGT AAT GGA TT | 58 | 35 |
| Â | R- CCC ACA AAA ATA CAC CCA TA | Â | Â |
TMS1/ASC RT-PCR | F- GGA CGC CTT GGC CCT CAC CG | 65 | 35 |
| Â | R- GGC GCG GCT CCA GAG CCC TG | Â | Â |
TMS1/ASC CHIP | F- GAG TCG GGA GAC CAG AGT GGA | 68 | 50 |
| Â | R- ACA GCA GCT TCA GCTT GAA CTT CTT G | Â | Â |
β-actin | F- ACC ATG GAT GAT GAT ATC GC | 60 | 30 |
| Â | R- ACA GGC TGG GGT GTT GAA G | Â | Â |
GAPDH | F- CCC CAC ACA CAT GCA CTT ACC | 65 | 50 |
| Â | R- CCT AGT CCC AGG GCT TTG ATT | Â | Â |