Figure 7

a, b : Mutant HIF-1α P582S has enhanced protein levels in SCC cells possessing TSC1/TSC2 mutations. Full length, HA-tagged wild-type HIF-1α and P582S mutant in pcDNA3 plasmids were transfected into SCC-4a and SCC-9 cells. a: Immunoblot of HIF-1α with anti-HAepitope-tag antibody. Anti-actin antibody was used as control for normalization of protein samples. b:Relative optical density of Western blots bands of HIF-1α wild-type and P582S mutant in SCC-1a and SCC-9 cells. 10 ng, 50 ng, and 100 ng plasmid: a, b, c and g, h, i (WT), 10 ng, 50 ng, and 100 ng plasmid: d, e, f and j, k, l (P582S mutant). (*, P < 0.05)