Down-regulation of c-Myc by U0126 in non-muscle tumor cell lines. A. Total lysates from the indicated non-muscle tumor cell lines untreated (-) or treated (+) with 10 μM U0126 for indicated times were analysed by immunoblotting with anti-c-Myc, anti-pospho-c-Myc, anti-ERKs and anti pospho-ERKs. B. Total lysates from untransformed C2C12 and NIH3T3 were analysed by immunoblotting with anti-c-Myc, anti-ERKs and anti pospho-ERKs. α-tubulin expression shows the loading of samples. Similar results were obtained in two experiments.