Effects of U0126 on non-muscle tumor cell lines proliferation and anchorage-independent growth. A. The histogram shows the number of cells from tumor cell lines and, as control, from untransformed C2C12 and NIH3T3, cultured for 4 days with (+) or without (-) U0126. The data shown are the mean ± s.e.m. of triplicates of a representative experiment. B. Tumor cells left untreated (C) or treated with U0126 were tested for growth in soft agar. Colonies were photographed after 14 days. Similar results were obtained in two experiments.