Specific down-regulation of XIAP with RNA interference enhances the sensitivity of canine tumor cell-lines to TRAIL and doxorubicin

Table 1 Nucleotide Sequences of Canine Specific Primers for Real-Time Quantitative PCR.

Gene	Primer	Sequence (5'-3')	Tm (°C)	Product size (bp)	Accession number
GAPDH	Forward	TGT CCC CAC CCC CAA TGT ATC	58	100	AB038240
	Reversed	CTC CGA TGC CTG CTT CAC TAC CTT
HPRT	Forward	AGC TTG CTG GTG AAA AGG AC	56	100	L77488/L77489
	Reversed	TTA TAG TCA AGG GCA TAT CC
XIAP	Forward	ACT ATG TAT CAC TTG AGG CTC TGG TTT C	54	80	AY603038
	Reversed	AGT CTG GCT TGA TTC ATC TTG TGT ATG
c-IAP1	Forward	AGG CGT CCC CGT GTC CGA GAG	68	96	XM_858260
	Reversed	TAG CAT CAG GCC GCA GCA GAA GC
c-IAP2	Forward	AGG CCA ATG TAA TTA ATA AAC AGG A	62	94	DQ223014
	Reversed	AAC TAA GAC AGT ATC AAT CAG TTC TCT C
Smac/DIABLO	Forward	AGC AGA AGC TGC ATA TCA AAC TGG AG	62	90	XM_534661
	Reversed	ACT TCC TGC ACC TGC GAC TTC AC
p53	Forward	GCC CCT CCT CAG CAT CTC ATC	67	100	NM_001003210
	Reversed	GGC TCA TAA GGC ACC ACC ACA C
Caspase 3	Forward	ATC ACT GAA GAT GGA TGG GTT GGT	58	140	AB085580
	Reversed	GAA AGG AGC ATG TTC TGA AGT AGC ACT
Bcl-2	Forward	TGG AGA GCG TCA ACC GGG AGA TGT	61	87	AB116145
	Reversed	AGG TGT GCA GAT GCC GGT TCA GGT
p27KIP	Forward	CGG AGG GAC GCC AAA CAG G	60	90	AY455798
	Reversed	GTC CCG GGT CAA CTC TTC GTG
CCND1	Forward	ACT ACC TGA ACC GCT	56	151	AY620434
	Reversed	CGG ATG GAG TTG TCA

ISSN: 1476-4598