Skip to main content
Figure 4 | Molecular Cancer

Figure 4

From: The tumor suppressor DAL-1/4.1B and protein methylation cooperate in inducing apoptosis in MCF-7 breast cancer cells

Figure 4

Hypomethylation modulates apoptosis in MCF-7 Cl27 cells. A. Western blot analysis with the anti-asymmetric dimethylarginine antibody ASYM24 (Upstate Biochemical) shows significant reduction in the methylation of proteins in MCF-7 Cl27 cells when treated with 30 μM AdOX for 48 hours. B. TUNEL and global caspase activation assays show that AdOX-associated hypomethylation specifically increases the percentage of apoptotic cells in the presence of DAL-1/4.1B protein. Grey bars show the percentage of apoptotic cells as measured by TUNEL. Black bars represent the percentage of global caspase activation.

Back to article page