Figure 2
From: Overexpression of Eag1 potassium channels in clinical tumours
Expression of Eag1 RNA in peripheral tissues is restricted. Real-time PCR records on cDNA from different human RNAs. The average fluorescence obtained in three experiments (± standard error) is plotted against cycle number. The dotted line indicates the threshold values used to determine positive signals. A clear signal can be detected after 21 cycles only in brain (red diamonds), while testis (dark green circles) and adrenal glands (light green triangles) required several more cycles of amplification for the signal to reach threshold. The rest of the tissues were negative. The Inset shows the control amplification obtained simultaneously on the same samples using the human transferrin receptor (TFR) as a template to demonstrate RNA integrity.