Figure 4

Inhibition of proliferation of sarcoma cell lines by imipramine or siRNAs. Soft tissue sarcoma cells (rhabdomyosarcoma – TE-671, A-204 and firbosarcoma – HT-1080, Hs633t) were transfected with siRNAs against Eag1 or were exposed to 10 μM imipramine for 96 hours prior to performing the MTT assay. Data are relative values compared to proliferation in the presence of vehicle and presented as mean ± standard error. Statistical analysis compared imipramine with non-treated cells, and siRNA-Eag1 and -GAPDH against scrambled siRNA molecules, p values are shown.