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Figure 2 | Molecular Cancer

Figure 2

From: Sensitive detection of human papillomavirus type 16 E7-specific T cells by ELISPOT after multiple in vitro stimulations of CD8+ T cells with peptide-pulsed autologous dendritic cells

Figure 2

IFN-γ production by an HPV type 16 E7 peptide-specific HLA-A2+ T cell clone shows that peptide-pulsed mature DC efficiently bind and present the E7 peptide to T cells. HPV-16 E7-peptide-pulsed or non-pulsed mature DC were used as stimulator cells. Mature DC were co-incubated with a HPV-16 E7-specific CD8+ CTL clone. After 6 h, IFN-γ released in the co-culture was determined by IFN-γ ELISA. The T cell clone produced 2364 ± 28 pg/mL IFN-γ upon E7 peptide-pulsed mature DC stimulation compared to 83 ± 21 pg/mL IFN-γ upon control stimulation (p-value = 0.001).

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Molecular Cancer

ISSN: 1476-4598