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Table 1 Analysis of HPV-16 E7 antigen-specific IFN-γ secretion by means of the IFN-γ ELISPOT assay

From: Sensitive detection of human papillomavirus type 16 E7-specific T cells by ELISPOT after multiple in vitro stimulations of CD8+ T cells with peptide-pulsed autologous dendritic cells

DONOR

Fifth stimulation Positive cultures/total cultures

Percentage of antigen-specific spot forming cells (SFC) in positive wells

1

2/48

0.03–0.08 [0.06]

2

3/48

0.05–0.06 [0.05]

3

6/48

0.02–0.04 [0.03]

4

7/48

0.03–0.18 [0.08]

5

2/2

0.12–0.14 [0.13]

  1. Responses were considered positive if a minimum of 15 SFC were counted after the control had been subtracted and if the ratio of SFC in positive wells to that in control wells was > 3. The range of the percentage of antigen-specific SCF is also given, with the mean percentage between square brackets, calculated as the ratio between the number of spots in a positive well and the total number of T cells in that well.