Table 1 mRNA custom designed primer sequences for polymerase chain reaction analysis of angiogenic endothelial cells isolated from SCCVII mouse model neoplasms.
Gene | Sense sequence | Anti-sense sequence | ||
|---|---|---|---|---|
GADPH | P1 | 5'-ACCACAGTCCATGCCATCAC-3' | P2 | 5'-TCCACCACCCTGTTGCTGTA-3' |
Beta Actin | P1 | 5'-CGTGGGCCGCCCTAGGCACCA-3' | P2 | 5'-TTGGCCTTAGGGTTCAGGGGGG-3' |
CD31 | P1 | 5'-GTGCTCTATGCAAGCCTCCA-3' | P2 | 5'-TTCGAGGTGGTGCTGATGTC-3' |
P3 | 5'-ATGGAATTCCCCATTGAGGC-3' | P4 | 5'-GATTTCAAACTTGGGAGTGG-3' | |
P5 | 5'-TTCTGAACTCCAACAGCGAG-3' | P6 | 5'-AGGCTCAAGGGAGGACACTT-3' | |
P7 | 5'-TGCCGAAGGCCCAAAGAAGA-3' | P8 | 5'-GCTCAGACCTTAGGAAACCG-3' | |
Keratin 19 | P1 | 5'-TGGGCAGATTGTTGTTATGGA-3' | P2 | 5'-TCAGGGCAGTAATTTCCTCC-3' |
P3 | 5'-CCTGGCTGCAGATGACTTCA-3' | P4 | 5'-AGACGGAGGCCCGTTATGGA-3' | |
P5 | 5'-TAAAACTTCCACCGCGGACT-3' | P6 | 5'-TTCCTATAGCTATCGCCAGA-3' | |
Alpha V | P1 | 5'-CTCTGAATCTGCCGTCAGT-3' | P2 | 5'-GCCAAGTGCTTGCAGATCAC-3' |
P3 | 5'-GTGATGTTAGTGGTGACTAG-3' | P4 | 5'-AAGGTTGACCTCGCCGAAAG-3' | |
Beta 3 | P1 | 5'-CTGTTACAATATGAAGAATG-3' | P2 | 5'-TTTTCATCACATACTGTAGC-3' |
P3 | 5'-ACTGTGCCTGCCAGGCCTTT-3' | P4 | 5'-TCCTTGGGGCTGCACTCTTC-3' |