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Table 1 Effect of SMC3-deficiency on zebrafish development.

From: SMC3 knockdown triggers genomic instability and p53-dependent apoptosis in human and zebrafish cells

Treatments

Embryos no.

Apoptotic Phenotype (%)

(A) Uninjected

  

1 nl Danieau buffer

60

0%

(B) SMC3 Morpholino-antisense

  

smc3-MO (1 ng)

60

45%

smc3-MO (2 ng)

60

56%

smc3-MO (4 ng)

60

86%

smc3-MO (8 ng)

60

100%

smc3-mmMO (8 ng)

60

5%

(C) zfSMC3 rescue

  

smc3-MO (4 ng)/smc3 mRNA (250 pg)

60

15%

(D) p53 Morpholino-antisense rescue

  

p53-MO (4 ng)

60

0%

smc3-MO (4 ng)/p53-MO (4 ng)

60

0%

  1. Embryos at 1–2 cell stage were injected in the yolk with 1–3 nl of MO or mRNA to achieve the indicated dose. Each injection session consisted of 2–3 treatment groups of 30 embryos each and several experiments were performed to reach the sample size indicated. Embryos serving as the untreated group were injected with 1 nl of Danieau buffer. Embryos receiving a dual treatment, were first received smc3-MO and after randomization, half was injected with p53-MO or smc3-RNA. At 6 hpf the unfertilized eggs were removed and the developing embryos analyzed at 15 hpf. Embryos showing necrosis in the head were classified as apoptotic.
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Molecular Cancer

ISSN: 1476-4598