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Figure 2 | Molecular Cancer

Figure 2

From: MUC1 gene overexpressed in breast cancer: structure and transcriptional activity of the MUC1 promoter and role of estrogen receptor alpha (ERα) in regulation of the MUC1 gene expression

Figure 2

Expression of the MUC1 isoform specific mRNA in human mammary epithelial cells detected by RT-PCR. Total RNA from human MCF-7, T47D and MDA-231 cells and RNA from mouse DA3 cells stably transfected with MUC1/SEC (lane – SEC), MUC1/TM (lane – TM) and MUC1/Y (lane – Y) cDNA (positive controls) were extracted and RT-PCR amplification of the MUC1 isoform specific fragments were performed. Lanes 1, 4, 7, 10, 13, 16 and SEC – PCR performed with MUC1/SEC specific primers; lanes 2, 5, 8, 11, 14, 17 and TM – PCR performed with MUC1/TM specific primers; lane 3, 6, 9, 12, 15, 18 and Y – PCR performed with MUC1/Y specific primers.

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