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Figure 2 | Molecular Cancer

Figure 2

From: Regulation of angiogenesis and invasion by human Pituitary tumor transforming gene (PTTG) through increased expression and secretion of matrix metalloproteinase-2 (MMP-2)

Figure 2

MMP-2 secretion and expression. A: Zymography analysis. Conditioned medium from HEK293 cells transiently or stably transfected with PTTG cDNA or pcDNA3.1 vector was collected. MMP-2 and MMP-9 levels in the medium were determined by gelatin zymography. Both active (68 kDa) and latent (72 kDa) forms of MMP-2 are shown. B: Graphical representation of the data shown in A. Data represents mean ± SEM from three independent experiments. C: ELISA assay. Levels of MMP-2 and MMP-9 (ng/ml) in conditioned medium collected from HEK293 cells transfected with PTTG cDNA or pcDNA3.1 vector. Data represent mean ± SEM from three independent experiments. ** = p < 0.05. D: Measurement of MMP-2 functional activity using Direct Quenching (DQ) fluorescence conjugate collagenase analysis. DQ assay was performed as described in materials and methods. Activity of collagenase was approximately 2.5 fold higher in CM from HEK293 cells transfected with PTTG cDNA as compared to CM collected from cells transfected with pcDNA3.1 vector. Addition of MMP-2 blocking antibody (MMP2BA) completely blocked the collagenase activity. Data represent the mean ± SEM of three independent experiments performed in triplicate. ** = p < 0.05.

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Molecular Cancer

ISSN: 1476-4598