Figure 7From: Regulation of angiogenesis and invasion by human Pituitary tumor transforming gene (PTTG) through increased expression and secretion of matrix metalloproteinase-2 (MMP-2)Expression of PTTG and MMP-2 in tumors and normal tissues. Tumors and other tissues were collected from nu/nu mice that developed tumors on injection of HEK293 cells constitutively expressing PTTG. A: Zymography analysis of tumor and normal tissues lysates. B: Semi-quantitative RT/PCR analysis of PTTG and MMP-2 expression using specific primers for PTTG and MMP-2 genes. GAPDH was used as an internal control. C: MMP-2 functional in situ fluorescence collagenase activity assay. The data shown are representative of three independent experiments.Back to article page