Flow cytometric histogram overlay of fluo-3 loaded PMNL. Representative plots of fMLP or C5a stimulated PMNL from normal donors (A and B) and CML patients (C and D). (a) isotype, (b) unstimulated, stimulated with fMLP or C5a for (c) 10 sec, (d) 30 sec, (e) 60 sec, (f) calcium ionophore A23187, (g) EGTA and (h) MnCl2. X-axis indicates fluorescence channel No. and Y-axis indicates No. of cells.