Flow cytometric estimation of extent of stimulation of [Ca2+]
in PMNL. Fluo-3 loaded CML and normal PMNL were stimulated with fMLP (10-8 M) or C5a (10-9 M), followed by treatment with calcium ionophore A23187, EGTA and MnCl2. X-axis indicates the treatment given to the cells and Y-axis indicates ratios of average [Ca2+]i levels in stimulated cells to the [Ca2+]i levels in unstimulated cells, i.e. basal level.