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Table 1 Different gene mRNA expression on CD133+ cells compared to those on autologous CD133- cells *

From: Analysis of gene expression and chemoresistance of CD133+ cancer stem cells in glioblastoma

  

No. 66

No. 377

No. 1049

Section

Gene name

CD133-

CD133+

CD133-

CD133+

CD133-

CD133+

A

CD90

1

15.6 ± 0.66

1

12.8 ± 0.94

1

13.5 ± 0.75

 

CD44

1

5.7 ± 0.48

1

2.5 ± 0.22

1

2.8 ± 0.19

 

CXCR4

1

337.8 ± 29.2

1

251.5 ± 22.1

1

264.9 ± 22.9

 

Nestin

1

21.4 ± 1.25

1

23.2 ± 1.65

1

22.1 ± 1.54

 

MSI

1

84 ± 7.6

1

75.4 ± 7.03

1

53.5 ± 6.2

 

MELK

1

1351 ± 95.8

1

467.7 ± 40.5

1

514.6 ± 45.6

 

GLI-1

1

46 ± 3.8

1

43 ± 4.5

1

49 ± 5.9

 

PTCH

1

16 ± 1.48

1

13.5 ± 0.85

1

14.3 ± 1.24

B

MGMT

1

32.4 ± 2.5

1

34.7 ± 2.9

1

56.3 ± 4.2

 

BCRP1

1

6.5 ± 0.43

1

4.3 ± 0.25

1

4.8 ± 0.24

 

SIRT1

1

4.9 ± 0.34

1

4.2 ± 0.26

1

5.4 ± 0.29

 

FLIP

1

294 ± 25.5

1

157.6 ± 14.2

1

145.6 ± 13.7

 

BCL-2

1

13.9 ± 0.95

1

4.9 ± 0.54

1

3.8 ± 0.54

 

BCL-XL

1

5.6 ± 0.39

1

3.2 ± 0.16

1

2.5 ± 0.14

 

cIAP1

1

39.0 ± 3.5

1

4.3 ± 0.53

1

5.6 ± 0.65

 

cIAP2

1

3 ± 0.25

1

1.9 ± 0.12

1

1.7 ± 0.14

 

XIAP

1

21.9 ± 2.2

1

9.7 ± 0.68

1

10.3 ± 0.91

 

NAIP

1

12.1 ± 0.75

1

6.4 ± 0.43

1

4.5 ± 0.62

 

Survivin

1

1.6 ± 0.08

1

2.3 ± 0.18

1

2.4 ± 0.18

 

BAX

1

0.33 ± 0.03

1

0.49 ± 0.06

1

0.21 ± 0.05

  1. * Total RNA was extracted from both CD133+ and CD133- fraction after FACS sorting from three primary cultured cells (No. 66, 377 and 1049). Different gene mRNA expression was measured by real-time qPCR. The relative mRNA expression of different gene in CD133+ cells was presented as the fold increase compared to that of autologous CD133- cells (see Methods). Data was presented as Mean ± SD of three independent experiments.