Skip to main content
Figure 10 | Molecular Cancer

Figure 10

From: P53 and p73 differ in their ability to inhibit glucocorticoid receptor (GR) transcriptional activity

Figure 10

N-terminal sequences affect p53 and p73 inhibition of GR. A, Schematic of p53 and p73 N-terminal swap mutants in which the N-terminal TAD and PRD have been swapped. B, Saos-2 cells were transfected with the GR-responsive luciferase reporter GR-luc (200 ng), GR DNA (100 ng), and increasing amounts DNA encoding the indicated p53:p73 protein (50 – 1000 ng of each DNA). Transfected cells were treated with Dex (100 nM) as described earlier, and luciferase activity in transfected cell lysates was determined. Fold change in GR-luc activity is plotted (+/- s.e.m.) from multiple experiments compared to the activity of GR-luc alone, whose value is considered 1.0. C, Representative immunoblots show the level of each protein.

Back to article page