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Figure 3 | Molecular Cancer

Figure 3

From: P53 and p73 differ in their ability to inhibit glucocorticoid receptor (GR) transcriptional activity

Figure 3

p53 and p73 differ in their ability to inhibit GR transcriptional activity. A, Saos-2 cells were transfected with the GR-responsive luciferase reporter GR-luc (200 ng), and DNA encoding GR (100 ng), and increasing amounts of HA p53 or HA p73β DNA (250, 500, 1000 ng) as indicated. 24 hrs after transfection, cells were untreated or treated with dexamethasone (100 nM) for an additional 20 hrs. Luciferase activity in transfected cell lysates was determined and relative luciferase activity (RLU) is plotted from triplicate experiments (+/- s.e.m.). Lower Representative immunoblot shows p53, p73, GR, and MDM2 expression levels in this experiment. B, Saos-2 cells were transfected with the GR responsive reporter GR-luc (200 ng) and DNA encoding GR (100 ng), and increasing amounts of Flag p53 or Flag p73β DNA (50, 100, 250, 500, 750, 1000 ng) as indicated. 24 hrs after transfection, cells were untreated or treated with dexamethasone (100 nM) for an additional 20 hrs. Luciferase activity in transfected cell lysates was determined and are plotted. The fold change in GR-luc activity is plotted (+/- s.e.m.) from multiple experiments compared to the activity of GR-luc alone, whose value is considered 1.0. Lower, Representative immunoblot shows GR, p53, p73, and MDM2 protein levels in transfected cell lysates.

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