Figure 6

C-terminal sequences in p53 and p73 affect their ability to inhibit GR. A, Schematic of p53 and p73 C-terminal deletion mutants (ΔC) and C-term swap mutants. B, Saos-2 cells were transfected with the GR-responsive luciferase reporter GR-luc (200 ng), GR DNA (100 ng), and increasing amounts DNA encoding the indicated p53:p73 protein (50 – 1000 ng of each DNA). Transfected cells were treated with Dex (100 nM) as described earlier, and luciferase activity in transfected cell lysates was determined. Fold change in GR-luc activity is plotted (+/- s.e.m.) from multiple experiments compared to the activity of GR-luc alone, whose value is considered 1.0. C, Representative immunoblots show the level of each protein.