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Figure 9 | Molecular Cancer

Figure 9

From: P53 and p73 differ in their ability to inhibit glucocorticoid receptor (GR) transcriptional activity

Figure 9

C-terminal sequences affect p53 and p73 inhibition of GR. A, Schematic of p53 and p73 C-terminal swap mutants in which the oligo and C-term have been swapped. B, Saos-2 cells were transfected with the GR-responsive luciferase reporter GR-luc (200 ng), GR DNA (100 ng), and increasing amounts DNA encoding wt p53 or p73, or the indicated p53:p73 C-terminal swap mutants (50 – 1000 ng of each DNA). Transfected cells were treated with Dex (100 nM) as described earlier, and luciferase activity in transfected cell lysates determined. Fold change in GR-luc activity is plotted (+/- s.e.m.) from multiple experiments compared to the activity of GR-luc alone, whose value is considered 1.0. C, Representative immunoblots show the level of each protein.

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