HT1080 cells were allowed to invade for 48 hours in the presence or absence of the specified lipid. siRNA treated HT1080 cells (Luciferase, MT1-MMP, or a control scrambled MT1-MMP) invaded in the presence of 1 μM LPA. Invasion distances were obtained with Metamorph® software by measuring pixel distance at each time point (five measurements per time point of duplicate or triplicate experiments.) Rates of invasion were calculated using linear regression analysis. R2 values denote the predictive potential of distance given time where 1.0 equals an exact correlation (* denotes separate experiment).