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Table 1 LPA and S1P accelerate MT1-MMP-mediated HT1080 invasion in 3D collagen matrices

From: Tumor cell invasion of collagen matrices requires coordinate lipid agonist-induced G-protein and membrane-type matrix metalloproteinase-1-dependent signaling

Condition

Rate of Invasion (μm/hr)

R2

DMEM

4.930

0.8977

LPA

13.971

0.9986

S1P

15.672

0.9777

LPA + S1P

14.941

0.9916

LPA + GM6001

1.010

0.6971

Luciferase*

13.873

0.9997

Scrambled MT1-MMP*

13.532

0.9988

si MT1-MMP*

5.366

0.9584

  1. HT1080 cells were allowed to invade for 48 hours in the presence or absence of the specified lipid. siRNA treated HT1080 cells (Luciferase, MT1-MMP, or a control scrambled MT1-MMP) invaded in the presence of 1 μM LPA. Invasion distances were obtained with Metamorph® software by measuring pixel distance at each time point (five measurements per time point of duplicate or triplicate experiments.) Rates of invasion were calculated using linear regression analysis. R2 values denote the predictive potential of distance given time where 1.0 equals an exact correlation (* denotes separate experiment).