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Figure 2 | Molecular Cancer

Figure 2

From: Valproic acid and butyrate induce apoptosis in human cancer cells through inhibition of gene expression of Akt/protein kinase B

Figure 2

Deactivation of Akt by valproic acid and butyrate. (A) Equal amounts of whole cell extract (50 μg) were used to examine the levels of endogenous Akt and phospho-Akt in the HeLa cells treated with valproic acid (VPA, 2 mM) or sodium butyrate (NaB, 5 mM) for 16 or 24 hours. The blot was then stripped and reprobed with a SRC antibody for protein loading controls. (B) Quantitative analysis of the Akt blots is expressed as fold variations compared to untreated control after being normalized to the loading controls. Error bars represent standard deviations of three independent experiments. (C) The quantification of phospho-Akt Western blots was performed as described for panel B.

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Molecular Cancer

ISSN: 1476-4598