Figure 3

Lentiviral transduction of Y79 with BE-GFP-Rb. A. Structure of BE-GFP-Rb^WT. Rb and GFP are expressed from a bidirectional promoter consisting of EF1α enhancer + promoter (light blue) and a minimal CMV promoter (dark blue) that requires a heterologous enhancer for activity [56]. B. Flow cytometric analysis of GFP expression in transduced cultures at 56 h after infection. Numbers indicate the percent GFP+ cells. C. Y79 cell cycle profiles 60 h after mock infection or transduction with the indicated BE-GFP viruses. D. Western analysis of Rb, p27, and α-actin 60 h after mock infection (lane 1) or infection with the indicated BE-GFP viruses (lanes 2–6).