Figure 1From: Histone deacetylase inhibitor, Trichostatin A induces ubiquitin-dependent cyclin D1 degradation in MCF-7 breast cancer cellsEffect of TSA on GFP-Cyclin D1 localization in MCF-7 cells. Cells were transfected with expression vectors encoding wild type GFP-Cyclin D1 (GFP-Cyclin D1WT). 24 h after transfection, cells were treated with TSA (1 μM) alone or in the presence of MG132 (50 μM) or leptomycin B (LMB) (10 ng /ml) for 6 h. Cells were fixed in ice cold methanol for 5 min, counterstained with DAPI and examined by fluorescence microscopy. Cells with predominantly cytoplasmic GFP-cyclin D1 are indicated by grey arrows.Back to article page