Figure 1From: Identification of candidate methylation-responsive genes in ovarian cancerRT-PCR confirmatory analysis of representative genes shown by microarray analysis to be upregulated after 5-aza-dC treatment of OVCAR-3 cell line. Expression results from the microarray analysis were confirmed by RT-PCR for CAV1, SFN, EMP3, CST6, CXCL6 and MAGEA3. All the genes showed induction of expression after 5-aza-dC treatment (AZA) relative to untreated cells (PBS). + and - indicate the presence or absence of reverse transcriptase during cDNA synthesis. GAPDH was used as an endogenous control.Back to article page