Table 3 Primers used for RT-PCR and Sodium Bisulfite sequencing
From: Identification of candidate methylation-responsive genes in ovarian cancer
Gene | Primer | Sequence | Size | Tm | Ref. |
|---|---|---|---|---|---|
CAV1 | RT-PCR_F | 5'-CGACCCTAAACACCTCAACGATG-3' | 278 | 55°C | [15] |
| Â | RT-PCR-R | 5'-GCAGACAGCAAGCGGTAAAACC-3' | Â | Â | Â |
|  | MOD_F | 5'-TGTGTATTTTGTAAATATGGTATAATTTG-3' | 537 | 58°C | [15] |
| Â | MOD_R | 5'-CATTTTCCCTACTCTAAACCAC-3' | Â | Â | Â |
SFN | RT-PCR_F | 5'-GTGTGTCCCCAGAGCCATGG-3' | 161 | 60°C | [23] |
| Â | RT-PCR-R | 5'-ACCTTCTCCCGGTACTCACG-3' | Â | Â | Â |
CST6 | RT-PCR_F | 5'-AAGACCAGGGTCACTGGAGA-3' | 163 | 60°C | [12] |
| Â | RT-PCR-R | 5'-CGGGGACTTATCACATCTGC-3' | Â | Â | Â |
|  | MOD_F | 5'-GGTTGGAATGTTGTAGTGGT-3' | 430 | 55°C | [12] |
| Â | MOD_R | 5'-CCCCAACAACAAATACCAA-3' | Â | Â | Â |
MAGEA3 | RT-PCR_F | 5'-TGGAGGACCAGAGGCCCCC-3' | 725 | 68°C | [16] |
| Â | RT-PCR-R | 5'-GGACGATTATCAGGAGGCCTGC-3' | Â | Â | Â |
|  | MOD_F | 5'-AGATTTGGTTTGAGGGGAGTAGAAGT-3' | 405 | 60°C | [16] |
| Â | MOD_R | 5'-AACCC(G/A)ACAACAAAAACAACACTAAA-3' | Â | Â | Â |
EMP3 | RT-PCR-F | 5'-TGCTCTCCCTCATTCTCTGCTGTC-3' | 274 | 60°C |  |
| Â | RT-PCR-R | 5'-CGCTTCCGTAGGTGGATGTAGATG-3' | Â | Â | Â |
|  | MOD-F | 5'-TAGTATATATTGAGAGGAGGAGAG-3' | 340 | 62°C | [17] |
| Â | MOD-R | 5'-CTTCCCAAACTACTACATTCCCA-3' | Â | Â | Â |
CXCL6 | RT-PCR-F | 5'-CCTGAAGAACGGGAAGC- 3' | 135 | 60°C |  |
| Â | RT-PCR-R | 5'-GACTGGGCAATTTTATGATG-3' | Â | Â | Â |
|  | MOD-F | 5'-GAGGGATGAATGTAGATAAAGGGAGT-3' | 616 | 60°C |  |
| Â | MOD-R | 5'-AACTTCCAAATCCAAACAAACTTACTT-3' | Â | Â | Â |
GAPDH | RT-PCR-F | 5'-GAAATCCCATCACCATCTTCCAG-3' | 312 | 56°C |  |
| Â | RT-PCR-R | 5'-ATGAGTCCTTCCACGATACAAAAG-3' | Â | Â | Â |