Ovarian dysgerminomas are characterised by frequent KIT mutations and abundant expression of pluripotency markers

Table 2 DNA sequence status of KIT exon 17 and expression of KIT in OGCTs

Patient ID	Histology of core	Bilateral	KIT protein expression	KIT mutation in codon 816, exon 17
PT-09	DG (w/GB)		-	GAC to TAC, Asp to Tyr
PT-16	DG		+	GAC to TAC, Asp to Tyr
PT-49	DG		++	GAC to CAC, Asp to His
PT-52	DG		++	GAC to GTC, Asp to Val
PT-58	DG		-	GAC to GTC, Asp to Val
PT-04	DG	Bilateral	++	wt
	GB	Bilateral	++	wt
PT-32	DG	Bilateral	++	wt
	YST	Bilateral	-	wt
PT-35	DG (left ovary)	Bilateral	++	wt
	DG (right ovary)	Bilateral	++	wt
PT-57	GB	Bilateral	M	wt
	DG (left ovary)	Bilateral	++	wt
	DG (right ovary)	Bilateral	++	wt
PT-03	DG (w/GB)		++	wt
	EC		-	wt
	GB		-	wt
PT-15	DG		M	wt
PT-19	DG		-	wt
PT-27, 39	DG		++	wt
PT-40	DG (w/GB)		++	wt
PT-48	DG		+	wt
PT-53	CC		-	wt
	DG		+	wt
PT-28	DG		++	wt
	YST		-	wt
PT-08	YST		M	wt
PT-20	YST		+	wt
PT-25,33,43,45,54,55	YST		-	wt
PT-06,10,18,23,31,34,44,46,47,51	IT		-	wt
PT-12, 22	IT		M	wt
PT-36, 37	YST, IT		-	wt
PT-11, 59, 56	SCC		-	wt

Staining intensity = '++': >50%, of tumour cells stained; '+': staining in approximately 20–50% of tumour cells; '+-': few cells stained; '-': no positive cells detected. Abbreviations: DG = dysgerminoma; GB = gonadoblastoma; YST = yolk sac tumour; EC = embryonal carcinoma; CC = choriocarcinoma; IT = immature teratoma; OGCT = ovarian germ cell tumour; PT = patient; SCC = small cell carcinoma; M = missing; wt = wild type.

ISSN: 1476-4598