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Figure 4 | Molecular Cancer

Figure 4

From: Factor interaction analysis for chromosome 8 and DNA methylation alterations highlights innate immune response suppression and cytoskeletal changes in prostate cancer

Figure 4

DNA methylation analysis of EPB41L3 and GADD45A. A: Bisulfite sequencing of the EPB41L3 promoter in selected cell lines and prostate tissue samples. Du145, PC3, 22Rv1, and LNCaP: prostate cancer cell lines. T: tumor tissues; N: benign tissues, UP: normal urothelial cells. B: Examples of Methylation-specific PCR for EPB41L3. M: primers specific for methylated promoter sequence, U: primers specific for unmethylated promoter sequence. T: prostate carcinoma tissue, N: benign prostate tissue, Bl: blood leukocyte DNA as unmethylated control, Du: Du145 DNA as methylated control. C: Effect of 5-aza-dC and SAHA on GADD45A expression. For each cell line from left to right: untreated (white bars), 5-aza-dC (horizontal stripes), SAHA (vertical stripes), 5-aza-dC + SAHA (black bars). D: Bisulfite sequencing of GADD45A in selected cell lines and prostate tissue samples. Du145, PC3, 22Rv1, and LNCaP: prostate cancer cell lines. T: tumor tissues; N: benign tissues

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Molecular Cancer

ISSN: 1476-4598