Figure 6
From: Mechanisms of osteopontin and CD44 as metastatic principles in prostate cancer cells
Analysis of the effects of OPN over expression on cell morphology and RANKL expression in PC3 cell lines. A. Indicated PC3 cell lines were photographed using a phase contrast microscope. An increase in multinucleated giant cells was observed in PC3/OPN cells (magnification × 200). B. Immunoblotting analysis of RANKL expression in PC3 cell lines Immunoblotting analysis in protein lysates made from the indicated cell lines was performed with an antibody to RANKL. PC3/OPN cells express greater level of RANKL that PC3 cells. Results shown are a representation of three independent experiments. Bottom panel shows normalization with GAPDH.