ELAC1 downregulation in metastases. We used real-time RT-PCR to validate the expression of five genes with altered expression in metastases. ELAC1 was validated as a downregulated gene in colorectal cancer, with a particular downregulation in the liver metastases and carcinomatoses. Values are here normalized according to values from normal colon mucosa before log2-transformation. Red and blue colored circles denote results from individual samples using real time RT-PCR and microarray experiments, respectively. N, normal colon mucosa; P, primary carcinoma; L, liver metastasis; C, carcinomatosis.