A schematic representation of the full-length TFPI-2 gene and a novel TFPI-2 splice variant. (A) The full-length TFPI-2 gene consists of 5 exons (designated by roman numerals) and 4 introns (designated by letters). (B) The novel splice variant reported here is generated by splicing of complete exon II, 15 nucleotides of exon III (AAAGTTCCCAAAGTT), 6 nucleotides of (GAATCC) intron C, 7 nucleotides of exon IV (GCAAAAG) and complete exon V. (B) The complete (289 bp) asTFPI-2 cDNA sequence is shown in 5' to 3' orientation. (C) The nucleotide sequences at the splice site junctions that result in the generation of asTFPI-2. The arabic numbers in the TFPI-2 gene diagram (A) correspond to the splice site locations. Splice site numbers 1–3 and 8–9 have normal consensus splice sites, whereas sites 4–7 and 10 are non-consensus splice sites. The intronic sequences are presented in lower case letters, while the exonic sequence is presented in upper case letters.