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Figure 1 | Molecular Cancer

Figure 1

From: HHV-8 encoded LANA-1 alters the higher organization of the cell nucleus

Figure 1

Comparison of expression levels of virus encoded and exogeneously introduced LANA-1 in the nucleus of HHV-8 positive BCBL-1 body cavity lymphoma (left) and HHV-8 negative MCF7 breast carcinoma cell (middle). Immunofluorescence staining (green) using human anti-LANA-1 serum detected by FITC conjugated mouse anti-human immunoglobulins. The images are Z axis projections of stacks of 10 optical sections, 0.5 micrometer apart, captured from identically stained and processed nuclei using an automated wide field fluorescence microscope. The counterstaining of BCBL-1 DNA with Hoechst 33258 (blue) is shown for easier orientation. The 3D projection of the plot of the measured staining intensity (right) illustrates that the nuclear foci of latently infected cells contain comparable amount of LANA-1 to the transiently transfected ones.

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