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Figure 9 | Molecular Cancer

Figure 9

From: HHV-8 encoded LANA-1 alters the higher organization of the cell nucleus

Figure 9

3D reconstitution of the triple-stained nuclei shows that LANA-1 staining and the 3MK9H3 positive remnants of the heterochromatic chromocenters are non-overlapping. See the full rotational image sequences in the supplementary material [see Additional file 1]. The selected middle sections (5–9) demonstrate the extensive peripheral localization of the chromocenters. High magnification representation of a single section shows mutual avoidance of 3MK9H3 staining and LANA-1 distribution. 3D reconstitution of LANA-1 transfected nuclei also reveals well-defined nuclear areas that are devoid of LANA-1 staining. The identity and molecular content of these areas are currently unknown but they apparently include the nucleoli and the chromocentric remnants. The 3 dimensional digital cast of the LANA-1 negative areas in relation to 3mK9H3 and DNA staining is shown as image sequence in the supplementary material [see Additional file 2]. (LANA-1 – red, 3MK9H3 – green, DNA – blue).

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