Skip to main content
Figure 2 | Molecular Cancer

Figure 2

From: A novel small molecule that selectively inhibits glioblastoma cells expressing EGFRvIII

Figure 2

Examples of EGFRvIII and control cell growth responses to small molecule diversity set compounds. a) Most compounds showed no effect on either cell line. The two matched cell lines with no drugs (red circle = 897 and red square = 898) have a growth curve similar to the matched lines grown with a drug (light blue circle = 897, dark blue square = 898). Under normal circumstances, the 898 cells with EGFRvIII (square symbols), will eventually overtake the 897 mutant free cells (circle symbols) within the wells of the 96 well plate. b) Those compounds with a general toxic effect are apparent because both 897 and 898 will stop growing in response to drug treatment compared to cells without drug. c) Single inhibition of the 898 mutant cells as shown indicates a possible hit on the primary screen. d-f: Example of secondary screen results showing concentration effect on EGFRvIII containing cells. d) 4 μM concentration. e) 0.05 μM concentration f) 0.025 μM concentration. Each point represents an average of four experiments. Growth curves for mutant containing cells (898) are labeled as follows: dark blue square is with compound; red square is without compound. Isogenic cells without EGFRvIII (897) are labeled as follows: light blue circle is with compound; red circle is without compound.

Back to article page