Figure 3

a) Initial growth response curve for 897 and 898 treated with NSC-154829. Growth curves for mutant containing cells (898) are labeled as follows: dark blue square, with compound; red square, without compound. Isogenic cells without EGFRvIII (897) are labeled as follows: light blue circle, with compound; red circle, without compound. Each point represents an average of duplicate experiments. b) Initial screens at 2 μM showing the selective effect of NSC-154829 on D54E cells, demonstrating efficacy across different genetic backgrounds. *** p < 0.0001. Further screening (see Table 1) would demonstrate significantly different IC₅₀s for the D54 and D54E cell lines when treated with NSC-154829. c) Structure of NSC-154829, showing purine backbone.