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Figure 6 | Molecular Cancer

Figure 6

From: A novel small molecule that selectively inhibits glioblastoma cells expressing EGFRvIII

Figure 6

Western blot showing effect of 2 μM NSC-154829 on expression of phosphorylated Akt (serine 473; P-Akt) in both 897 and 898 cell lines (a), or on expression of p53 and p21 in the 897/898 and D54/54E cell lines under normal conditions (10% serum) or serum starvation (0.5% serum). a) Lanes 5–6 demonstrate the effect of serum starvation on the expression of P-Akt in both cell lines; this increased expression is diminished by treatment with 2 μM NSC-154829 (lanes 7 and 8). b) 897 and 898 cells were grown under conditions of either 10% serum (lanes 1–4) or 0.5% serum (lanes 5–8). Under conditions of 10% serum, exposure to 2 μM NSC-154829 has minimal effect on expression of p53, except for a mild decrease in the wild-type cells (897) under serum starvation conditions (lane 7). p21 expression increases for both the wild-type and mutant cells under both normal and serum starvation conditions; although the increase appears greater for the mutant cells (compare lanes 2 and 4). c) Effects of NSC-154829 on p53 and p21 in the D54 and 54E cell lines. Under conditions of 10% serum, there is minimal effect on p53 and a mild decrease in p21 signaling by NSC-154829. Serum starvation (0.5% serum) combined with NSC-154829 treatment produces an increase in p21 in both cell lines, mutant greater than wild-type (compare lanes 6 and 8), but does not appear to affect p53 expression.

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