Skip to main content
Figure 4 | Molecular Cancer

Figure 4

From: LEDGF/p75 has increased expression in blasts from chemotherapy-resistant human acute myelogenic leukemia patients and protects leukemia cells from apoptosis in vitro

Figure 4

Effects of LEDGF/p52 splice variants on spontaneous and daunorubicin-induced apoptosis. Flp-In 293 cells were cultured in medium without (A), or with 0.5 μM daunorubicin (DNR) for 16 hours (B, C). The cells were transfected with control vector (pcDNA3.1), p52b (with exon 6), with p52Δ1, p52bΔ1 or p52bΔE6 (all of which lack exon 6), or with LEDGF/p75317-530, i.e. the part of the LEDGF/p75 gene (exons 10–15) that do not overlap with p52. In panel C, cells were co-transfected with full length LEDGF/p75 and p52Δ1, p52bΔ1, p52bΔE6, or C-LEDGF/p75317-530. Scoring of cell viability and other experimental conditions are as described in the legend to Fig. 5.

Back to article page

Molecular Cancer

ISSN: 1476-4598