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Figure 3 | Molecular Cancer

Figure 3

From: O6-methylguanine-DNA methyltransferase is downregulated in transformed astrocyte cells: implications for anti-glioma therapies

Figure 3

MGMT is downregulated in immortalized and transformed NHA cells. (A) The expression of MGMT mRNA in NHA (parental; lane 1), NHA/T (lane 2), NHA/TS (lane 3), NHA/TSR (lane 4), and NHA/TSRA cells (lane 5) were analyzed by semi-quantitative RT-PCR. The levels in flank xenografts derived from the NHA/TSR and NHA/TSRA cells were also tested (lanes 6 and 7). (B) Protein extracts (10 μg) from NHA (lanes 1–4) and TIG3 cells (lanes 5–7) infected with retroviral vectors expressing indicated genes (T, hTERT: S, SV40ER: R, H-RasV12; A; myrAKT) were analyzed by immunoblotting.

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Molecular Cancer

ISSN: 1476-4598