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Figure 6 | Molecular Cancer

Figure 6

From: O6-methylguanine-DNA methyltransferase is downregulated in transformed astrocyte cells: implications for anti-glioma therapies

Figure 6

VPA inhibits cell growth in transformed human astrocyte cells. (A) Dose-dependent anti-proliferative effects of VPA were shown. NHA/TSRA cells were treated with 0, 0.5 and 1.0 mM VPA for 14 days. Cell numbers were counted every other day and results were expressed in the mean of two independent experiments. (B) NHA/TSRA cells, treated (0.5 mM) and untreated (0 mM) with VPA for two weeks, were subjected to soft-agar colony formation assay. 2 × 104 cells were plated, incubated at 37°C for 21 days and stained with 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide. Note VPA was not included in the assay media. (C) Protein extracts (10 μg) from NHA/TSRA cells treated with VPA were analyzed by immunoblotting using indicated antibodies.

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