PTTG expression in different experimental and human prolactinomas in relation to dopaminergic control of lactotropes

Table 2 Gene-specific primers for mouse PTTG and GAPDH, and human PTTG, Estrogen receptor α and GAPDH used in this study

Gene	Sequence (Forward)	Nucleotide location	Sequence (reverse)	Nucleotide location	Product size bp	Cycles	Temp annealing
Mouse PTTG	CAGCCGTGCCTAAAGCCAGC	463–482	GATAGAAAGGGTGTCTTCAGAG	799–820	358	35	58°C
Mouse GAPDH	CTCACGGCAAATTCAACGG	195–213	CTTTCCAGAGGGGCCATCCA	603–622	428	26	55°C
Human PTTG1	CGATGCCCCACCAGCCTTACC	195–215	CAAGCTCTCTCTCCTCGTCAAGG	489–511	317	40	57°C
Human ER-α	TCCAGCACCCTGAAGTCTCT	1749–1768	TCTCCAGCAGCAGGTCATAG	1970–1989	241	40	63°C
Human GAPDH	GCCAAAAGGGTCATCTC	271–290	GCAGGGATGATGTTCTGGAG	532–551	281	32	57°C

ISSN: 1476-4598