Expression of LIMK1 in prostate tumors. A: Panel 1 demonstrates the strong staining of basal cells of benign prostatic glands with LIMK1 (solid arrow) (X400). In panel 2, there is a very weak staining of a poorly differentiated prostatic adenocarcinoma (X400). Moderate staining of scattered apparent smooth muscle cells can be identified in the stroma immediately adjacent to malignant cells (solid arrow). B: Panel 1 shows very weak staining of the cytoplasm of prostate cancer with no nuclear staining (X400). Panel 2 demonstrates no staining to strong nuclear staining of prostatic adenocarcinoma. The cytoplasmic staining varies from weak to strong (X400). Panel 3 demonstrates very strong nuclear staining for LIMK1 for prostatic adnocarcinoma and moderate to strong cytoplasmic staining (X400). Panel 4 demonstrates very strong cytoplasmic staining for LIMK1 for prostatic adenocarcinoma. There is also variable nuclear staining of prostatic adenocarcinoma for LIMK1, which varies from no staining to moderate staining. C. Comparison of immunostaining scores between cytoplasmic and nuclear staining. D. Receiver operating characteristic (ROC) analysis of cyoplasmic (solid line) and nuclear staining (dotted line) in relation to incidence of metastasis or no metastasis. ROC curve for nuclear staining showing a shift towards the y-axis indicates a better association of nuclear staining with the incidence of metastasis compared to cytoplasmic staining.