Figure 5
Blocking of Cy5-labeled RGD peptide accumulation in HEK293(β3) s.c. tumors by coinjection with unlabeled RGD peptide. (A) Tumor-bearing mice received i.v. injection of 10 nmol Cy5-RAFT-c(-RGDfK-)4 alone (a), or coinjected with 1200 nmol cRGD (b) or 300 nmol RAFT-c(-RGDfK-)4 (c). (B) Tumor-bearing mice received i.v. injection of 10 nmol Cy5-cRGD alone (a), or coinjected with 1200 nmol cRGD (b) or 300 nmol RAFT-c(-RGDfK-)4 (c). Left panel: representative fluorescence images at 2 hr p.i.; Right panel: kinetics of fluorescence intensities in tumors. T, K and Int indicate tumor, kidney and intestine, respectively. Data are expressed as means ± SD (n = 2–4).