Figure 6

A-B. TNF-α-induced p21 does not have antiapoptotic function. (A) LN-18 cells were grown on coverslips and transfected with p21 siRNA oligonucleotides (100 nM) for 24 hr. Control and transfected cells were treated with TNF-α (10 ng/ml) for 3 hr. Cells were stained with p21 antibody followed by secondary antibody labeled with Cy3. DAPI was used for nuclear staining (blue fluorescence), merged images show nuclear localization of p21 (Magnification 63×). (B) Effect of TNF-α on viability in p21 siRNA transfected cells done by MTT assay. The graph represents mean +/- SD of 3 experiments done in duplicates.