Figure 7

A-B. p21 but not p27 is involved in TNF-α induced inhibition of proliferation. (A) LN-18 cells were transfected with p21 siRNA oligonucleotides (100 nM) or (B) p27 antisense (As) oligonucleotides (200 nM) for 6 hr and cultured as monolayers or spheroids for 18 hr. Cells were treated with TNF-α (10 ng/ml) for 24 hr, and tritiated thymidine incorporation assay was done as described in Fig. 1. * p < 0.05 inhibition of proliferation with TNF-α in transfected versus untransfected cells. The data is representative of three experiments done in triplicates.