Figure 4From: Characterization of thimet oligopeptidase and neurolysin activities in B16F10-Nex2 tumor cells and their involvement in angiogenesis and tumor growthHPLC analysis of neurotensin (NT) degradation by B16F10-Nex2 supernatant. Neurotensin (20 μM) was incubated for 1 h at 37°C with recombinant TOP (A), neurolysin (B) or B16F10-Nex2 supernatant (D) in 50 mM Tris-HCl, pH 7.4. The reaction products were separated by HPLC. HPLC profile of melanoma supernatant without NT is shown on panel C. The neurotensin fragments were determined by mass spectrometry.Back to article page