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Figure 5 | Molecular Cancer

Figure 5

From: Characterization of thimet oligopeptidase and neurolysin activities in B16F10-Nex2 tumor cells and their involvement in angiogenesis and tumor growth

Figure 5

HPLC analysis of neurotensin (NT) degradation by B16F10-Nex2 supernatant in the presence of bestatin. Neurotensin (20 μM) was incubated for 1 h at 37°C with recombinant TOP (A), neurolysin (B) or B16F10-Nex2 supernatant (D) in the presence of bestatin in 50 mM Tris-HCl, pH 7.4. The reaction products were separated by HPLC. HPLC profile of melanoma supernatant in the presence of bestatin without NT is shown on panel C. The neurotensin fragments were determined by mass spectrometry.

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Molecular Cancer

ISSN: 1476-4598